![]() We verified the performance of 16S-FASAS on both mock and fecal samples. 16S-FASAS consists of data quality control, de novo assembly, annotation, and visualization modules. Herein, we developed software named 16S-FASAS (16S full-length amplicon sequencing data analysis software) for 16S-FAS-NGS data analysis, which provided high-fidelity species-level microbiome data. However there is a lack of a data analysis suite that can help process and analyze the synthetic long read data. The 16S-FAS-NGS (16S rRNA full-length amplicon sequencing based on a next-generation sequencing platform) technology can generate high-quality, full-length 16S rRNA gene sequences using short-read sequencers, together with assembly procedures. BMC bioinformatics, 2016, 17(1): 135.The full-length 16S rRNA sequencing can better improve the taxonomic and phylogenetic resolution compared to the partial 16S rRNA gene sequencing. Sensitivity and correlation of hypervariable regions in 16S rRNA genes in phylogenetic analysis. Use of 16S rRNA and rpoB genes as molecular markers for microbial ecology studies. Case R J, Boucher Y, Dahllöf I, et al. ![]()
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